Journal: Frontiers in Genetics

Article Title: Identification and validation of a seven-gene metastasis-associated prognostic model in breast cancer

doi: 10.3389/fgene.2026.1770418

Figure Lengend Snippet: Prognostic gene expression in cell lines. (A) RTN1-A, RTN1-B and RTN1-C expression in tumor (n = 1,080) and para-tumor (n = 98) samples from TCGA-BRCA. (B) Ratios of RTN1-A/RTN1, RTN1-B/RTN1 and RTN1-C/RTN1 in tumor and paratumor samples from TCGA-BRCA. (C) mRNA levels of prognostic genes in MCF-10A, MCF-7, SKBR3, MDA-MB-231 and MDA-MB-468 cells (n = 4). (D) Electrophoretogram of the PCR products of CXCL14 (171 bp), RTN1-A (134 bp), and RTN1-C (78 bp). The data are presented as the means ± SDs. P values were calculated via Student’s unpaired t-test. *P < 0.05; **P < 0.01; ***P < 0.01; ****P < 0.0001.

Article Snippet: MCF-10A cells were maintained in MCF-10A cell complete medium (Procell, CM-0525) (DMEM/F12 + 5% house serum + 20 ng/mL EGF + 0.5 μg/mL hydrocortisone +10 μg/mL insulin + 1% NEAA + 1% P/S).

Techniques: Gene Expression, Expressing